[Myotox] Myotoxin discussion

[Borries Demeler]

Hello all,
I am forwarding the information from Bruno to the mailing list so we
have a record there, including the attached papers so you all have
access to them.

In addition: Tony/Mike and Paul: Amy has ~ 100 ul of pure myotoxin-II at
21 mg/ml (high concentration) available. It is in phosphate buffer as
far as I know. We could get that to you. If you need more, I am sure
Bruno could arrange another shipment.

I also discussed the project with Harmen. He had a lot of interesting
ideas with respect on how to prepare nanodisks and membranes, and how 
to check the interaction with Mytoxin-II with the membrane. He has been
working on a very similar project testing destruction of mitochondrial
membranes by cytochrome-C, Paul, this is the project we wanted to pursue
with you. Harmen sent some samples to you guys earlier, not sure if they
were ever analyzed, but perhaps we could pick this up again as well?
(and Paul, feel free to add this into the discussion for the CFI, since
it would be another excellent example for the collaboration between our
groups and justify the CMP-MAS probe).

Harmen, I'm going to let you chime in and share your ideas on synthetic
belts, and various types of lipids that could be used for making
appropriate nanodiscs. Perhaps you could also find the source for the 
muscle tissue membrane lipids you talked about, as well as some other 
more readily available membrane systems we could try.

Bruno indicated that most membranes would be affected by the toxicity
to some degree (what's different about the membranes of the snake's 
toxin-producing glands/toxin-conducting tissues??)

Thanks, -b.



----- Forwarded message from Bruno Lomonte <bruno.lomonte at ucr.ac.cr> -----

Date: Fri, 22 Nov 2019 12:01:25 -0600
From: Bruno Lomonte <bruno.lomonte at ucr.ac.cr>
To: "Montina, Tony" <tony.montina at uleth.ca>, "Hazendonk, Paul" <paul.hazendonk at uleth.ca>, "Opyr, Michael"
	<michael.opyr at uleth.ca>, Borries Demeler <demeler at gmail.com>
Subject: Re: Research Collab Meeting
User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:60.0) Gecko/20100101 Thunderbird/60.9.1

Thanks so much to all for your interest in exploring possibilities to
study the interaction of the myotoxin II with membranes.

here attached is the paper in which some phospholipid compositions
were studied using liposomes, back in 1992

there was also a follow up with an interesting observation about
potential auto-acylation of the myotoxin (also attached, 1995)

codes for the crystal structe of the protein are:  1CLP and 1Y4L

some info about the active region of the toxins here attached too (2001)

happy to provide any further info, and of  course, protein, if you
find this project is viable and interesting

special thanks to Borries for bridging our interests

all the best,

Bruno
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