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that would be great Amy and Sebastien, it will be much easier to
ship within the US than from here<br>
<br>
thanks and best regards!<br>
<br>
Bruno<br>
<br>
+++++++++++++++++++++<br>
<br>
<br>
<br>
<div class="moz-cite-prefix">On 10/31/2022 11:52 AM, Henrickson, Amy
wrote:<br>
</div>
<blockquote type="cite"
cite="mid:YT2PR01MB4525BB7FE413B8226ACE9091EF379@YT2PR01MB4525.CANPRD01.PROD.OUTLOOK.COM">
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
<style type="text/css" style="display:none;">P {margin-top:0;margin-bottom:0;}</style>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif;
font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255,
255, 255);" class="elementToProof">
Hello Sebastien and Bruno, <br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif;
font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255,
255, 255);" class="elementToProof">
<br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif;
font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255,
255, 255);" class="elementToProof">
I also have quite a bit of toxin left and would be able to send
it to Sebastien. Please let us know if/or when you need more
sample.
<br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif;
font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255,
255, 255);" class="elementToProof">
<br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif;
font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255,
255, 255);" class="elementToProof">
Cheers, <br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif;
font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255,
255, 255);" class="elementToProof">
Amy Henrickson <br>
</div>
<hr style="display:inline-block;width:98%" tabindex="-1">
<div id="divRplyFwdMsg" dir="ltr"><font style="font-size:11pt"
face="Calibri, sans-serif" color="#000000"><b>From:</b> Myotox
<a class="moz-txt-link-rfc2396E" href="mailto:myotox-bounces@biophysics.uleth.ca"><myotox-bounces@biophysics.uleth.ca></a> on behalf of BRUNO
LOMONTE <a class="moz-txt-link-rfc2396E" href="mailto:BRUNO.LOMONTE@ucr.ac.cr"><BRUNO.LOMONTE@ucr.ac.cr></a><br>
<b>Sent:</b> Sunday, October 23, 2022 10:59 AM<br>
<b>To:</b> Myotoxin-II discussion
<a class="moz-txt-link-rfc2396E" href="mailto:myotox@biophysics.uleth.ca"><myotox@biophysics.uleth.ca></a><br>
<b>Subject:</b> Re: [Myotox] manuscript draft</font>
<div> </div>
</div>
<div style="font-size:10pt; font-family:Verdana,Geneva,sans-serif">
<div style="border:solid #646464 1.0pt; padding:2.0pt 2.0pt
2.0pt 2.0pt; background:#FFEB9C">
<p class="x_MsoNormal" style="line-height:12.0pt; margin:5px"><b><span
style="font-size:10.0pt; color:#9C6500"></span></b><span
style="font-size:10.0pt; color:black">Caution: This email
was sent from someone
<b>outside of the University of Lethbridge</b>. Do not
click on links or open attachments unless you know they
are safe. Suspicious emails should be forwarded to
<a class="moz-txt-link-abbreviated" href="mailto:phishing@uleth.ca">phishing@uleth.ca</a>.</span></p>
</div>
<br>
<div>
<p>Hi all,</p>
<p>I am on my way back from the IST congress in Abu Dhabi -
I believe there should be some toxin available to send it to
Sebastien (could you provide me with the address and
shipping details?)</p>
<p>best regards to all,</p>
<p>Bruno</p>
<p>+++++++++++++++</p>
<p><br>
</p>
<div id="x_signature">---<br>
<div class="x_pre" style="margin:0; padding:0;
font-family:monospace">Bruno Lomonte, PhD<br>
Instituto Clodomiro Picado<br>
Facultad de Microbiología<br>
Universidad de Costa Rica<br>
San José, Costa Rica</div>
</div>
<p><br>
</p>
<p id="x_reply-intro">El 2022-10-23 9:49 am, Borries Demeler
escribió:</p>
<blockquote type="cite" style="padding:0 0.4em;
border-left:#1010ff 2px solid; margin:0">
<div id="x_replybody1">
<div dir="ltr">
<div>Dear Sebastien,</div>
<div>Thanks for the corrections, I will apply them to
our manuscript.</div>
<div> </div>
<div>Regarding samples, Bruno is your source. Amy, I am
not sure how much we have left, but maybe there is
some available that can be sent to Sebastien,
otherwise, Bruno, could you supply some more to
Sebastien?</div>
<div> </div>
<div>Also, would everyone else who should be a co-author
please provide your affiliation details and
acknowledgments, if any.</div>
<div> </div>
<div>Thanks, -Borries</div>
<div dir="ltr"> </div>
<br>
<div class="x_v1gmail_quote">
<div class="x_v1gmail_attr" dir="ltr">On Fri, Oct 21,
2022 at 11:46 AM Sebastien Poget <<a
href="mailto:Sebastien.Poget@csi.cuny.edu"
rel="noreferrer" moz-do-not-send="true"
class="moz-txt-link-freetext">Sebastien.Poget@csi.cuny.edu</a>>
wrote:</div>
<blockquote class="x_v1gmail_quote" style="margin:0px
0px 0px 0.8ex; border-left:1px solid #cccccc;
padding-left:1ex">
<div class="x_v1msg6668595578802411008">
<div dir="ltr" style="font-size:12pt;
color:#000000; background-color:#ffffff;
font-family:Calibri,Arial,Helvetica,sans-serif">
<p>Dear Borries and all,</p>
<p>The manuscript looks promising, I'll be
curious to read it again when the NMR data are
added. I just noticedthat my affiliation in
the acknowledgements should be City University
of New York, not State University of NY (and
thanks for acknowledging receipt of the
lysolipids, I just wish that they would have
had more of an effect).</p>
<p>As far as the bilayer experiments go, I have
just recruited a new undergraduate who is
interested in continuing theses experiments
and go more in depth with checking detergent
and toxin concentrations to see if we can
induce membrane pore formation. We have a
little bit of toxin left, but if the
experiments show any promise, we would need
more. Is there any chance that we could obtain
another aliquot? And lastly, if you think that
it would be useful to obtain a mass spec of
exactly the batch of toxin you did the AUC on
(which, I assume, is what you sent us), I
could easily run that on our MALDI-TOF and
provide you with a spectrum.</p>
<p>Best,</p>
<p>Sebastien</p>
<p><br>
</p>
<p><br>
</p>
<div style="color:#212121">
<hr style="display:inline-block; width:98%">
<div
id="x_v1m_-5420361867440351162divRplyFwdMsg"
dir="ltr"><span style="font-size:11pt;
color:#000000;
font-family:Calibri,sans-serif"><strong>From:</strong>
Myotox <<a
href="mailto:myotox-bounces@biophysics.uleth.ca"
rel="noreferrer" moz-do-not-send="true"
class="moz-txt-link-freetext">myotox-bounces@biophysics.uleth.ca</a>>
on behalf of Ana Gisele da Costa Neves
Ferreira <<a
href="mailto:anagextra@gmail.com"
rel="noreferrer" moz-do-not-send="true"
class="moz-txt-link-freetext">anagextra@gmail.com</a>><br>
<strong>Sent:</strong> Thursday, October
20, 2022 8:30 AM<br>
<strong>To:</strong> Myotoxin-II
discussion<br>
<strong>Subject:</strong> Re: [Myotox]
manuscript draft</span>
<div> </div>
</div>
<div>
<div dir="ltr">Dear Borries,
<div>I'll send you my comments ASAP, sorry
for the delay.</div>
<div>Best regards,</div>
<div>AnaG</div>
</div>
<br>
<div class="x_v1gmail_quote">
<div class="x_v1gmail_attr" dir="ltr">Em
seg., 17 de out. de 2022 às 13:13,
Borries Demeler <<a
href="mailto:demeler@gmail.com"
rel="noreferrer"
moz-do-not-send="true"
class="moz-txt-link-freetext">demeler@gmail.com</a>>
escreveu:</div>
<blockquote class="x_v1gmail_quote"
style="margin:0px 0px 0px 0.8ex;
border-left:1px solid #cccccc;
padding-left:1ex">
<div dir="ltr">
<div>Dear colleagues,</div>
<div>So far I have only received
contributions from Bruno, I still
need the contributions from everyone
else who contributed to this work,
can you please finish them up and
send them to me?</div>
<div>Thanks again, -Borries </div>
</div>
<br>
<div class="x_v1gmail_quote">
<div class="x_v1gmail_attr" dir="ltr">On
Tue, Oct 4, 2022 at 5:26 AM Borries
Demeler <<a
href="mailto:demeler@gmail.com"
rel="noreferrer"
moz-do-not-send="true"
class="moz-txt-link-freetext">demeler@gmail.com</a>>
wrote:</div>
<blockquote class="x_v1gmail_quote"
style="margin:0px 0px 0px 0.8ex;
border-left:1px solid #cccccc;
padding-left:1ex">
<div dir="ltr">
<div>Hi Ana,</div>
<div>attached is the latest
version that incorporates
Bruno's additions. Sorry, I must
not have sent it to the list.</div>
<div>Regards, -Borries</div>
</div>
<br>
<div class="x_v1gmail_quote">
<div class="x_v1gmail_attr"
dir="ltr">On Mon, Oct 3, 2022 at
6:18 PM Ana Gisele da Costa
Neves Ferreira <<a
href="mailto:anagextra@gmail.com"
rel="noreferrer"
moz-do-not-send="true"
class="moz-txt-link-freetext">anagextra@gmail.com</a>>
wrote:</div>
<blockquote
class="x_v1gmail_quote"
style="margin:0px 0px 0px 0.8ex;
border-left:1px solid #cccccc;
padding-left:1ex">
<div dir="ltr">
<div dir="ltr">Hi Borries,
<div>I will wait for Bruno's
version to see if I can
contribute in any way.</div>
<div>Regards,</div>
<div>AnaG</div>
</div>
<br>
<div class="x_v1gmail_quote">
<div class="x_v1gmail_attr"
dir="ltr">Em qui., 29 de
set. de 2022 às 13:43,
Bruno Lomonte <<a
href="mailto:bruno.lomonte@ucr.ac.cr"
rel="noreferrer"
moz-do-not-send="true"
class="moz-txt-link-freetext">bruno.lomonte@ucr.ac.cr</a>>
escreveu:</div>
<blockquote
class="x_v1gmail_quote"
style="margin:0px 0px 0px
0.8ex; border-left:1px
solid #cccccc;
padding-left:1ex">
Thank you Borries, I am on
it and will get back to
you all soon<br>
best regards<br>
Bruno<br>
+++++++++++++++<br>
<br>
<br>
On 9/28/2022 4:01 PM,
Borries Demeler wrote:<br>
> Dear Colleagues,<br>
> attached is a draft
of our Myotoxin-II
manuscript - it still
needs <br>
> contributions from
others on this list. It
summarizes the AUC <br>
> component, and should
also include the NMR
results from Paul's group,
<br>
> as well as details
about the biology and
further background in the
<br>
> introduction from
Ana's and Bruno's group.
There may be some <br>
> additional
crystallography and mass
spec results that could be
<br>
> incorporated or
referenced, I leave that
up to you guys to decide.<br>
><br>
> I have provided a
odt, docx and pdf version.
I am not able to use <br>
> Microsoft Word, only
LibreOffice, hence there
may be some <br>
> conversion issues,
and your mileage may vary.<br>
><br>
> Feel free to delete
figures and equations and
just use track changes <br>
> to record your
changes and send back the
text you wish to add, <br>
> including author and
affiliation details. If
you want to send a <br>
> separate document I
can also integrate. Feel
free to add additional <br>
> endnotes (as plain
text, if possible).<br>
><br>
> Thanks, -Borries<br>
><br>
>
_______________________________________________<br>
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<br>
-- <br>
Bruno Lomonte, Ph.D.<br>
Instituto Clodomiro Picado<br>
Facultad de Microbiología<br>
Universidad de Costa Rica<br>
San José, COSTA RICA<br>
<br>
tel.of. (+506) 2511 7888<br>
<a
href="mailto:bruno.lomonte@ucr.ac.cr"
rel="noreferrer"
moz-do-not-send="true"
class="moz-txt-link-freetext">bruno.lomonte@ucr.ac.cr</a><br>
<br>
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<div><span
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font-family:tahoma,sans-serif; font-size:x-small">Ana Gisele da C. Neves
Ferreira</span></div>
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em Saúde
Pública</span></div>
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color:#0b5394;
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de Toxinologia</span></div>
<div><span
style="background-color:#ffffff;
color:#0b5394;
font-family:tahoma,sans-serif; font-size:xx-small">Instituto Oswaldo
Cruz - Fiocruz</span></div>
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05</span></div>
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Oswaldo Cruz - Fiocruz</span></div>
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color:#0b5394;
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font-size:xx-small">Pavilhao
Ozorio de Almeida, sala 05</span></div>
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color:#0b5394;
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Brasil, 4365 - Manguinhos</span></div>
<div><span
style="background-color:#ffffff;
color:#0b5394;
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</pre>
</blockquote>
<br>
<pre class="moz-signature" cols="72">--
Bruno Lomonte, Ph.D.
Instituto Clodomiro Picado
Facultad de Microbiología
Universidad de Costa Rica
San José, COSTA RICA
tel.of. (+506) 2511 7888
<a class="moz-txt-link-abbreviated" href="mailto:bruno.lomonte@ucr.ac.cr">bruno.lomonte@ucr.ac.cr</a></pre>
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