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Dear all,<br>
<br>
I was refreshing my memory on some older studies on a myotoxin that
is highly similar to Mt-II, and for which a paper on its interaction
with SDS had been presented in 2007 using circular dichroism, here
attached. Owing to my <b>very </b>limited understanding of
biophysical experiments, I do not know if this article could provide
us (or not) with any useful insights to the discussions and
interpretations of current experiments. <br>
<br>
The only other study on the SDS interaction is more recent (2019),
by a Japanese group, but I think we had already shared this few
months ago and actually sparked the initiatives to perform the SDS
experiments. Here I attach it in case some of us in the list did not
see it before.<br>
<br>
Looking forward to the discussion tomorrow<br>
<br>
Best regards to all<br>
<br>
Bruno<br>
<br>
<br>
PS: apologies for the personal yellow highlights in the files... I
guess they are all deletable<br>
<br>
+++++++++++++++<br>
<br>
<br>
<br>
<br>
<div class="moz-cite-prefix">On 4/1/2021 11:56 AM, Borries Demeler
wrote:<br>
</div>
<blockquote type="cite"
cite="mid:20210401175643.GA8305@asus.private.net">
<pre class="moz-quote-pre" wrap="">Dear all,
we have results back from the myotoxin-2 SDS titration experiments in
AUC. I feel these experiments provided some very interesting results and
are probably the most informative ones we have done so far. I also think
that they will be extremely helpful in designing the *correct* NMR
experiment. I went through the effort to write this up with figures,
please review carefully the attached document and read the explanations
I believe explain these results (which were quite unintuitive at first,
but now make sense to me). Then let's schedule another meeting and
discussion of next steps.
-Borries
</pre>
<br>
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<pre class="moz-quote-pre" wrap="">_______________________________________________
Myotox mailing list
<a class="moz-txt-link-abbreviated" href="mailto:Myotox@biophysics.uleth.ca">Myotox@biophysics.uleth.ca</a>
<a class="moz-txt-link-freetext" href="https://demeler7.uleth.ca/mailman/listinfo/myotox">https://demeler7.uleth.ca/mailman/listinfo/myotox</a>
</pre>
</blockquote>
<br>
<pre class="moz-signature" cols="72">--
Bruno Lomonte, Ph.D.
Instituto Clodomiro Picado
Facultad de Microbiología
Universidad de Costa Rica
San José, COSTA RICA
tel.of. (+506) 2511 7888
<a class="moz-txt-link-abbreviated" href="mailto:bruno.lomonte@ucr.ac.cr">bruno.lomonte@ucr.ac.cr</a></pre>
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