<div dir="ltr"><div>The slope could be indicative of the strength of binding. If the inflection point is nearly horizontal, it could mean the monomer jumps to a hexamer without intermediates and binds strongly. Vertical distributions mean pure species.diagonal distributions mean heterogeneity.</div><div><br></div><div>-Borries<br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Apr 1, 2021 at 4:22 PM Hazendonk, Paul <<a href="mailto:paul.hazendonk@uleth.ca">paul.hazendonk@uleth.ca</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Also what does the slope at the inflection point tell us?<br>
<br>
-----Original Message-----<br>
From: Myotox <<a href="mailto:myotox-bounces@biophysics.uleth.ca" target="_blank">myotox-bounces@biophysics.uleth.ca</a>> On Behalf Of Borries Demeler<br>
Sent: April 1, 2021 11:57 AM<br>
To: Myotoxin-II discussion <<a href="mailto:myotox@biophysics.uleth.ca" target="_blank">myotox@biophysics.uleth.ca</a>><br>
Subject: Re: [Myotox] myotoxin2-SDS titration<br>
<br>
Caution: This email was sent from someone outside of the University of Lethbridge. Do not click on links or open attachments unless you know they are safe. Suspicious emails should be forwarded to <a href="mailto:phishing@uleth.ca" target="_blank">phishing@uleth.ca</a>.<br>
<br>
<br>
Dear all,<br>
we have results back from the myotoxin-2 SDS titration experiments in AUC. I feel these experiments provided some very interesting results and are probably the most informative ones we have done so far. I also think that they will be extremely helpful in designing the *correct* NMR experiment. I went through the effort to write this up with figures, please review carefully the attached document and read the explanations I believe explain these results (which were quite unintuitive at first, but now make sense to me). Then let's schedule another meeting and discussion of next steps.<br>
<br>
-Borries<br>
_______________________________________________<br>
Myotox mailing list<br>
<a href="mailto:Myotox@biophysics.uleth.ca" target="_blank">Myotox@biophysics.uleth.ca</a><br>
<a href="https://demeler7.uleth.ca/mailman/listinfo/myotox" rel="noreferrer" target="_blank">https://demeler7.uleth.ca/mailman/listinfo/myotox</a><br>
</blockquote></div>