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<p class="MsoNormal"><span style="mso-fareast-language:EN-US">Can you do dynamic studies by varying the speed of centrifugation? I.e. the slope as a function of speed?<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span lang="EN-US">From:</span></b><span lang="EN-US"> Myotox <myotox-bounces@biophysics.uleth.ca>
<b>On Behalf Of </b>Borries Demeler<br>
<b>Sent:</b> April 1, 2021 4:37 PM<br>
<b>To:</b> Myotoxin-II discussion <myotox@biophysics.uleth.ca><br>
<b>Subject:</b> Re: [Myotox] myotoxin2-SDS titration<o:p></o:p></span></p>
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<span style="font-size:10.0pt;color:black">Caution: This email was sent from someone
<b>outside of the University of Lethbridge</b>. Do not click on links or open attachments unless you know they are safe. Suspicious emails should be forwarded to
<a href="mailto:phishing@uleth.ca">phishing@uleth.ca</a>.</span><o:p></o:p></p>
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<p class="MsoNormal">The s-value is reflective of the size of the molecule. A 5.5 s species is much bigger than a 2 s species. The y axis tells you the relative amount of species with a given s-value. It is a standard integral sedimentation distribution. I
am happy to have a zoom meeting to discuss further these interesting trends.<o:p></o:p></p>
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<p class="MsoNormal">-Borries<o:p></o:p></p>
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<p class="MsoNormal">On Thu, Apr 1, 2021 at 4:20 PM Hazendonk, Paul <<a href="mailto:paul.hazendonk@uleth.ca">paul.hazendonk@uleth.ca</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal">What is the best way to interpret these curves. I have not seen ultracentrifugation data since 1991. Should we be looking at the position of the inflection point?
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-----Original Message-----<br>
From: Myotox <<a href="mailto:myotox-bounces@biophysics.uleth.ca" target="_blank">myotox-bounces@biophysics.uleth.ca</a>> On Behalf Of Borries Demeler<br>
Sent: April 1, 2021 11:57 AM<br>
To: Myotoxin-II discussion <<a href="mailto:myotox@biophysics.uleth.ca" target="_blank">myotox@biophysics.uleth.ca</a>><br>
Subject: Re: [Myotox] myotoxin2-SDS titration<br>
<br>
Caution: This email was sent from someone outside of the University of Lethbridge. Do not click on links or open attachments unless you know they are safe. Suspicious emails should be forwarded to
<a href="mailto:phishing@uleth.ca" target="_blank">phishing@uleth.ca</a>.<br>
<br>
<br>
Dear all,<br>
we have results back from the myotoxin-2 SDS titration experiments in AUC. I feel these experiments provided some very interesting results and are probably the most informative ones we have done so far. I also think that they will be extremely helpful in designing
the *correct* NMR experiment. I went through the effort to write this up with figures, please review carefully the attached document and read the explanations I believe explain these results (which were quite unintuitive at first, but now make sense to me).
Then let's schedule another meeting and discussion of next steps.<br>
<br>
-Borries<br>
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