<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
</head>
<body>
<p>Hi all,</p>
<p>fully agree with Ana G!</p>
<p>Bruno</p>
<p><br>
</p>
<p>+++</p>
<p><br>
</p>
<div class="moz-cite-prefix">On 12/2/2020 20:08, Ana Gisele da Costa
Neves Ferreira wrote:<br>
</div>
<blockquote type="cite"
cite="mid:CAPgscZgPbMt4iH0pEp78ubr9GcpBron65EWcFpY2r6xwcwSUcg@mail.gmail.com">
<meta http-equiv="content-type" content="text/html; charset=UTF-8">
<div dir="ltr">
<div dir="ltr">Dear Borries,
<div>As Amy suggested before, I think the first ideia would be
to use the same experimental conditions assayed by
Matsui (attached paper). For SV-AUC experiments, the toxin
was dissolved in 20 mM Tris-HCl pH 8.0 and 200 mM NaCl at a
concentration of 1 mg/mL in the presence or absence of 1%
(w/v) SDS. SDS titration seems to be a good idea too. </div>
<div>Regarding NMR, what kind of experiment are you planning ?</div>
<div>Regards,</div>
<div>AnaG</div>
</div>
<br>
<div class="gmail_quote">
<div dir="ltr" class="gmail_attr">Em qua., 12 de fev. de 2020
às 18:02, Borries Demeler <<a
href="mailto:demeler@gmail.com" target="_blank"
moz-do-not-send="true">demeler@gmail.com</a>> escreveu:<br>
</div>
<blockquote class="gmail_quote" style="margin:0px 0px 0px
0.8ex;border-left:1px solid
rgb(204,204,204);padding-left:1ex">Dear Ana and Bruno,<br>
We are gearing up to start the AUC experiment in the
presence of SDS.<br>
Bruno, could you please go into your LIMS account at:<br>
<br>
<a
href="https://uslims.uleth.ca/uslims3_CCH/login.php"
rel="noreferrer" target="_blank" moz-do-not-send="true">https://uslims.uleth.ca/uslims3_CCH/login.php</a><br>
<br>
and update your LIMS project ("Myotoxin-II at high
concentration") and <br>
explain in the "Buffer Components" and "Experimental Design"
section <br>
how we should prepare the buffer and protein, i.e., what SDS<br>
concentrations to use, what buffer to use, what protein
concentrations<br>
to try. My suggestion would be to use a moderate
concentration (say, 0.5<br>
OD 280 nm), and perhaps do a SDS titration, but want to
defer to you<br>
or Ana in case you have a better idea.<br>
<br>
Amy is planning the experiments for the end of this week and
it would be <br>
good to get this info soon.<br>
<br>
On another topics:<br>
Paul and TOny: We have sufficient new material on hand to
run the<br>
baseline NMR experiments on Myotoxin now. Please send Amy
instructions<br>
for what concentration, buffer etc. you will need and we
will bring it<br>
to you. WHen can we run the NMR experiments?<br>
<br>
Thanks, -Borries<br>
_______________________________________________<br>
Myotox mailing list<br>
<a href="mailto:Myotox@biophysics.uleth.ca" target="_blank"
moz-do-not-send="true">Myotox@biophysics.uleth.ca</a><br>
<a href="http://demeler7.uleth.ca/mailman/listinfo/myotox"
rel="noreferrer" target="_blank" moz-do-not-send="true">http://demeler7.uleth.ca/mailman/listinfo/myotox</a><br>
</blockquote>
</div>
<br clear="all">
<div><br>
</div>
-- <br>
<div dir="ltr">
<div dir="ltr">
<div>
<div dir="ltr">
<div dir="ltr">
<div dir="ltr">
<div><span
style="color:rgb(11,83,148);font-family:tahoma,sans-serif;font-size:x-small">Ana
Gisele da C. Neves Ferreira</span><br>
</div>
<div><font style="background-color:rgb(255,255,255)"
size="1" face="tahoma, sans-serif"
color="#0b5394">Pesquisadora Titular em Saúde
Pública</font></div>
<div><font style="background-color:rgb(255,255,255)"
size="1" face="tahoma, sans-serif"
color="#0b5394">Chefe do Laboratorio de
Toxinologia</font></div>
<div><font style="background-color:rgb(255,255,255)"
size="1" face="tahoma, sans-serif"
color="#0b5394">Instituto Oswaldo Cruz - Fiocruz</font></div>
<div><font style="background-color:rgb(255,255,255)"
size="1" face="tahoma, sans-serif"
color="#0b5394">Pavilhao Ozorio de Almeida, sala
05</font></div>
<div><font style="background-color:rgb(255,255,255)"
size="1" face="tahoma, sans-serif"
color="#0b5394">Av. Brasil, 4365 - Manguinhos</font></div>
<div><font style="background-color:rgb(255,255,255)"
size="1" face="tahoma, sans-serif"
color="#0b5394">Rio de Janeiro - RJ </font></div>
<div><font style="background-color:rgb(255,255,255)"
size="1" face="tahoma, sans-serif"
color="#0b5394">21040-900 Brasil</font></div>
<div><font style="background-color:rgb(255,255,255)"
size="1" face="tahoma, sans-serif"
color="#0b5394">(+5521) 2562-1381 / 98801-5726</font></div>
<div style="color:rgb(136,136,136);font-size:12.8px"><font
size="1" face="tahoma, sans-serif"
color="#666666"><br>
<br>
<br>
</font></div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
<br>
<fieldset class="mimeAttachmentHeader"></fieldset>
<pre class="moz-quote-pre" wrap="">_______________________________________________
Myotox mailing list
<a class="moz-txt-link-abbreviated" href="mailto:Myotox@biophysics.uleth.ca">Myotox@biophysics.uleth.ca</a>
<a class="moz-txt-link-freetext" href="http://demeler7.uleth.ca/mailman/listinfo/myotox">http://demeler7.uleth.ca/mailman/listinfo/myotox</a>
</pre>
</blockquote>
<pre class="moz-signature" cols="72">--
Bruno Lomonte, Ph.D.
Instituto Clodomiro Picado
Universidad de Costa Rica
San José, 11501
COSTA RICA
<a class="moz-txt-link-abbreviated" href="mailto:bruno.lomonte@ucr.ac.cr">bruno.lomonte@ucr.ac.cr</a>
tel. +506 2511 7888
cel. +506 8392 0012
</pre>
</body>
</html>